Chitosan-bacterial cellulose patch of ciprofloxacin for wound dressing: Preparation and characterization studies

CACICEDO, MAXIMILIANO L.; PACHECO, GUILHERME; ISLAN, GERMAN A.; ALVAREZ, VERA A.; BARUD, HERNANE S.; CASTRO, GUILLERMO R.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

ELSEVIER SCIENCE BV

Año: 2020 vol. 147 p. 1136 - 1145

0141-8130

Biopolymeric blends based on bacterial cellulose (BC) films modified with low molecularweight chitosan (Chi) were developed for controlled release of ciprofloxacin (Cip).Biophysical studies revealed a compatible and cooperative network between BC and Chiincluding deep structural changes in the BC matrix shown by spectroscopic and thermalanalyses (SEM, roughness analysis, FTIR, XRD, TGA, mechanical properties and water vaportransmission rate). Incorporation of chitosan to BC matrix generated a thickening scaffoldwith high permeability to water vapor from 0.7 to 3.2 g mm/m2 h. Cip loaded onto the BCChifilm showed a hyperbolic release profile with a 30% decrease in antibiotic releasemediated by the presence of Chi. BC-Chi blend films containing Cip tested againstPseudomonas aeruginosa and Staphylococcus aureus showed a synergic effect of chitosanon Cip antimicrobial activity. Besides, in vitro studies revealed the lack of cytotoxicity of BCChi-Cip films in human fibroblasts