Seasonal study of the expression of the s-100 protein in pituitary pars nervosa of viscacha (Lagostomus maximus maximus).

MARIQUEO BETSABE; DÍAZ GUEVARA CARLA; ROSALES GABRIELA; FILIPPA VERÓNICA; PÉREZ EDITH; MOHAMED FABIAN

San Luis Capital

Congreso; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo.; 2019

Sociedad de Biología de Cuyo

The pars nervosa (PN) of the neurohypophysis is mainly constituted by pituicytes and other neuroglial cells such as microglial cells, astrocytes and oligodendrocytes, endothelial cells of blood vessels and amyelinic axons. The PN pituicytes express the S-100 protein that has a regulatory role within the cell and on different target cells. The aim of this work was to study the seasonal expression of S100 protein in pituitary PN of adult male viscachas (Lagostomus maximus maximus). The animals were captured in their habitat during the most representative months of their annual reproductive cycle. This cycle has three periods: reproductive (RepP), gonadal regression (RegP), and gonadal recovery (RecP). Four pituitary glands of each period were removed and processed for optical microscopy. The S-100 protein was used as markers of pituicytes and their expression was detected by immunohistochemistry. The primary antibody used was the rabbit polyclonal anti-S-100 protein. Immunohistochemical staining was performed using a streptavidin-biotin-peroxidase complex method with the 3,3‟-diaminobenzidine tetrahydrochloride (DAB) as the chromogen. A morphometric study was performed, and the percentage of immunopositive area for S-100 (% IA-S-100) was measured. The results obtained from these periods were statistically analyzed. Most of the pituicytes presented a cytoplasmic immunostaining pattern. However, some of them also exhibited nuclear staining. The nuclei were round, oval, and irregular in shape, with varying density of chromatin and an evident nucleolus. Numerous immunostained cytoplasmic processes in contact with blood vessels were observed.The % IA-S-100 varied throughout the annual reproductive cycle. The % IA during the RepP (13.09 ± 1.49) and RegP (9.77 ± 1.56) were significantly lower than in the RecP (25.61 ± 2.36; P