Expression of estrogen receptor and proliferation cellular nuclear antigen in pituitary pars distalis during the pregnancy of viscachas

ROSALES GABRIELA; RODRIGUEZ GRACIELA; FILIPPA VERÓNICA; MOHAMED FABIAN

Merlo, San Luis.

Congreso; XXXV Reunión Científica Anual. Sociedad de Biología de Cuyo.; 2017

Sociedad de Biología de Cuyo

The female viscacha is a monostral animal with an unusually long gestation period for a rodent. Three stages were classified: early (EP), middle (MP), and late pregnancy (LP). In previously studies variations of lactotrope cells were reported. The aim of this work was to analyze the expression of estrogen receptor (ERα) and proliferating cell nuclear antigen (PCNA) in pituitary of viscachas during pregnancy. Pituitaries of non-pregnant (NP) viscachas and pregnant from EP, MP and LP (n=4 per group) were processed for light microscopy. ERα and PCNA were detected by immunohistochemistry and quantified by image analysis. The colocalization of PCNAPRL was qualitatively analyzed. Serum estradiol was determined by radioimmunoassay. The immunostaining pattern for ERα was nuclear and cytoplasmic. Nuclear ER immunoreactivity (ERαn-ir) increased significantly in LP (NP: 0.25 ± 0.03%, EP: 0.11 ± 0.03%, MP: 0.19 ± 0.04%, LP: 0.62 ± 0.19%, p