LOCALIZATION OF ANDROGEN RECEPTORS IN PARS DISTALIS OF ADULT MALE VISCACHA DURING THE REPRODUCTIVE CYCLE

GODOY DAIANA.; ROSALES GABRIELA; FILIPPA VERÓNICA; MOHAMED FABIAN

San Luis Capital

Congreso; XXX Reunión Científica Anual. Sociedad de Biología de Cuyo.; 2012

Sociedad de Biología de Cuyo

Androgens are involved in the regulation of reproductive function. They bind to specific intracellular receptors, followed by shuttling of the activated androgen receptor (AR) to the cell nucleus, where it stimulates or represses gene transcription. The aim of this work was to study the expression of AR in pituitary PD of adult male viscachas by immunohistochemical techniques. These rodents were captured during the reproductive cycle: reproductive (n=4), gonadal regression (n=4) and gonadal recovery (n=4) periods. Pituitaries were processed for light microscopy and the AR was immunohistochemically identified using the antibody AR (N-20). Immunohistochemical results revealed that AR-ir was detected in the nuclei (ARn-ir) and/or cytoplasms (ARc-ir) of PD cells. However, the nuclear labeled was more intense in the caudal end during reproductive and gonadal regression periods. The higher nuclear immunolabeling was in the dorso-medial during gonadal recovery period. The cytoplasmic immunolabeling pattern was heterogeneous, some cells presented labeling in the nucleus periphery whereas other presented stippled cytoplasmic immunolabeling, during reproductive and gonadal regression periods. In the gonadal recovery period the cytoplasmic labeling was more intense and homogeneous in relation to the previous periods. These results demonstrated variations in the expression and localization of AR during the reproductive cycle, being probably more active during gonadal recovery of viscacha.