Characterization of MC540 photooxidated products and cytotoxicity in human carcinoma cell lines

CALZETTA N.L.; ALONSO, S. DEL V.; MARANZANA, E.S.B.

Mar del Plata

Congreso; 51 Reunión de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2015

SAIB, Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Merocyanine 540 (MC540) is a photosensitive dye that in the presence of light and oxygen showed antitumor activityagainst leukemia cell lines. The aim of this study is to characterize MC540 photoproducts (pMC540) and evaluatetheir cytotoxicity in vitro in human carcinoma cell lines. MC540 solutions were irradiated with fluorescent lampsduring 6 hs at 25°C. MC540 and pMC540 were characterized by UV-visible, fluorescence, FT-IR and massspectrometry. Human breast MCF-7, prostate PC-3 and colorectal Caco-2 carcinoma cells were plated in 96 wellplates and treated with different doses of pMC540 or MC540. Cell viability was determined by crystal violet, MTTand LDH assays after 4, 24 and 48 hs. Our results show that light irradiation of MC540 produces a mixture of at leasttwo major pMC540 that resulted to be dose-dependent cytotoxic (p0.05). None of the cell lines were susceptible to MC540 (p>0.05). We conclude that photooxidation breaks downMC540 generating at least two biologically active pMC540 which cytotoxicity is dependent on the cell line type. Ourhypothesis is that pMC540 interacts differentially depending on specific cell membrane constituents. Further assaysand analysis are ongoing to strengthen this hypothesis