(015) INVOLVEMENT OF TNF-Α IN THE ACTIVATION OF GAMMA DELTA T LYMPHOCYTES BY HUMAN GLOMERULAR ENDOTHELIAL CELLS EXPOSED TO SHIGA TYPE 2 TOXIN. ROLE IN THE PATHOGENESIS OF HEMOLYTIC UREMIC SYNDROME

DAVID ROSSO; ROSATO, MICAELA; FERNANDO DANIEL GOMEZ; ÁLVAREZ, ROMINA S.; SHIROMIZU, CAROLINA M.; CRISTINA IBARRA; MARIA MARTA AMARAL; CAROLINA JANCIC

Ciudad Autónoma de Buenos Aires

Congreso; Reunión Anual de Biociencia 2020; 2021

Sociedad Argentina de Investigación Clinica

The hemolytic uremic syndrome (HUS) associated with diarrhea,a consequence of Shiga toxin (Stx)-producing Escherichia coli infection, is a common cause of pediatric acute and chronic renalfailure. Stx type 1 and type 2 (Stx1 and Stx2) produced by thosebacteria are the main factors related with HUS that trigger kidneydamage. Stx2-producing strains are associated with severe casesof HUS in Argentina. gd T cells are a specialized subset of T lymphocytes, which act as early sensors of cellular stress and infection.They can exert cytotoxicity against infected and transformed cellsand produce cytokines and chemokines. In this work, we studiedthe activation of human peripheral gd T cells in response to humanglomerular endothelial cells (HGEC) exposed to Stx2 (0.01 ng/ml,24 h) or their conditioned medium. We analyzed CD69, CD107a,and perforin expression by flow cytometry; and cytokine productionby ELISA and intracellular staining in gd T cells after incubation withStx2-treated HGEC or their conditioned medium. We evaluated byconfocal microscopy, the interaction between gd T cells and HGECtreated or not with Stx2 and perforin distribution. As result, we observed an increase in TNF-α, IFN-γ, and cell interactions (p