Aquaporins can be involved in the swelling caused by Shiga toxin type 2 on HGEC and HK-2 cells.

FERNANDO DANIEL GOMEZ; JULIETA REPETTI; ROMINA ROMERO; FLAVIA SACERDOTTI; CRISTINA IBARRA; MARIA MARTA AMARAL

Ciudad Autónoma de Buenos Aires

Congreso; Reunión Anual de Biociencia 2020; 2021

Sociedad Argentina de Investigación Clinica

Hemolytic uremic syndrome related to Shiga toxin?producing Escherichia coli (STEC￾HUS) is the principal etiology of acute kidney injury in children in Argentina.Previously, we demonstrated that Shiga toxin type 2 (Stx2) damages human glomerular endothelial cells (HGEC) and HK-2 human proximal tubular epithelial cell line by inducingswelling and detachment. In this work, we analyzed cell volume changes of HGEC and HK-2 exposed or not to Stx2 or a hypoosmotic (HYPO) medium, in the presence or not of aquaporins (AQPs) inhibitors (mercuric chloride: HgCl2 and tetraethylammonium: TEA) or Eliglustat (EG), an inhibitor of Stx2 receptor (Gb3) synthesis. For controls, an Isosmotic (ISO) medium was used.Cells were grown on 12 well plates and pretreated for 30 minutes with HgCl2 (10 M) or TEA (100 M) or during 24 h with EG (10 M). Then, HGEC and HK-2 were incubated with Stx2 (50 M) for an additional 40 minutes. Cell volume was analyzed by light microscopy and cell area was measure by using Image J software. After Stx2 and HYPO medium treatments, a significant increase in the cell volume of HGEC (Stx2: 42%; HYPO: 36%, n= 3, p