Urolithins modulate endometriosis progression related genes in-vitro.

BILOTAS M; BOGGAVARAPU N; PONANDAI-SRINIVASAN S; GEMZELL-DANIELSSON K; MERESMAN G

Congreso; Endometriosis Online Congress, IVF worldwide online Congress; 2022

OBJECTIVE: Urolithins are a subfamily of metabolites generated by the human intestinal microbiota from ellagitannins and EA, which are polyphenols mainly found in red fruits. In previous studies, it has been demonstrated that they have anti-inflammatory, anticancer, antioxidant, antimicrobial and antiestrogenic effects. Moreover, we demonstrated the anti-proliferative, anti-migratory, anti-invasive and pro-apoptotic effects of Urolithin A (UA) and urolithin B (UB) in endometriosis in-vivo and in-vitro. In addition, it is well known that angiogenesis is one of the mayor process involved in endometriotic lesions establishment and progression. Taking these data into account, the aim of the present work was to evaluate the effect of UA and UB on the expression of a variety of genes related to angiogenesis, migration and invasion in-vitro. MATERIAL AND METHODS: 12z endometriotic cells were stimulated with UA, UB or vehicle (Basal) or with culture media alone (control) for 24 h. Afterwards, cells were collected, total RNA was extracted and gene expression was analyzed by quantitative real time RT-PCR using Taq-Man probes. RESULTS: UA decreased the expression of VEGF, RHOJ, IL1B, FGF2 and IL6 pro-angiogenic and proinflammatory genes (p