Preconcentration and determination of Pb at ultra-trace levels in bee products by magnetic ionic liquid based dispersive liquid-liquid microextraction coupled to electrothermal atomic absorption spectrometry

FIORENTINI, EMILIANO; BOTELLA, MARÍA B.; WUILLOUD, RODOLFO G.

Santiago de Chile

Conferencia; 6th IBEROAMERICAN MEETING ON IONIC LIQUIDS (IMIL 2021); 2021

Universidad de Santiago de Chile

In this work, a highly efficient dispersive liquid-liquid microextraction method based on MIL (MIL-DLLME) and detection by ETAAS was developed to determine Pb at ultra-trace levels in bee products. The analyte was chelated with 1,5-diphenylcarbazide in acidic medium (2 mol L-1 HCl) and was extracted by 150 µL of trihexyl(tetradecyl)phosphonium tetrachloromanganate (II) ([P6,6,6,14]2MnCl4). Subsequently, the extractant phase was separated by a magnet and an aliquot was injected into ETAAS. The optimal extraction conditions were established by multivariate optimization. Under optimal experimental conditions, a 97.0% extraction efficiency and a sensitivity enhancement factor of 107 were obtained. Detection limit (LOD) of 3 ng L-1 with a relative standard deviation of 3.6% (at 30 ng L-1 Pb, n=10) and a linear range of 10-3000 ng L-1 were obtained. This work reports the first application of the MIL [P6,6,6,14]2MnCl4 along with the DLLME technique for the determination of Pb at trace levels in different bee products, including honey, mead, honey vinegar and honey beer.