DYSBIOSIS ASSOCIATED TO AZITRHOMYCIN ADMINISTRATION

PIQUERAS VIRGINIA; JAIME CRISTIAN; GIAJ MERLERA GUILLERMO; VELEZ PABLO; BELAUS ANDREA; CORREA SILVIA

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

The gut microbiota is a complex community of microorganisms that plays an important role in the development and maintenance of the immune system and gastrointestinal functionality. Alterations in the microbiota composition (dysbiosis) could initiate or enhance autoimmune or inflammatory diseases. Among the factors that cause dysbiosis, the administration of antibiotics is one of the most frequent. In this work we evaluated the impact of acute administration of azithromycin (AZM) on the intestinal microbiota. Adult male C57BL/6 mice received AZM (50 mg /kg/day) in drinking water for 5 days and stool samples were collected to infers a microbial community structure based on analyses of terminal-restriction fragment length polymorphisms (T-RFLP) of 16S rRNA gen and density for flow cytometry (FC), samples of small and large intestine (SI and LI) to evaluate mucus by colorimetric method, luminal content to determine metabolic profiles by GC-MS and proximal and distal mesenteric lymph nodes (MLN) to evaluate CD3, CD4, CD8 and CD19 lymphoid subsets by FC. After the administration of AZM, the relative abundances of the main phyla showed modifications with an increase in Firmicutes (p