Hyperthyroidism increases milk immune cells and impairs offspring development in early lactation.

SANCHEZ MB; MORENO-SOSA MT; NEIRA FJ; SOAJE M; PIETROBON EO; FARIAS H; JAHN GA; VALDEZ SR; MACKERN OBERTI JP

Evento Virtual

Congreso; LXV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2020

Sociedad Argentina de Investigación Clínica

Hyperthyroidism (H) reduced milk ejection and quality, impairingmaternal behavior and mammary gland development. However, itremains unclear if H impacts in milk immune cells numbers. Our aimis to assess the influence of H on i) pup maturation and developmentii) prolactin secretion and iii) milk immune cells. For this purpose,10-12 weeks old Wistar rats were injected daily with T4 (0.25 mg/kg until day 18 of gestation, then 0.1 mg/kg until day 2 of lactationL2) to induce H or with vehicle in control group. Rats were mated 8days after starting T4 treatment and euthanized L2 (after ketamine/xylazine sedation and oxytocin stimulation for milking). Afterwards,milk and mammary gland samples, minced to reach single cell suspension,were dyed with fluorophore labeled mAbs (CD45+, CD3+,CD11b/c+) and analyzed by flow cytometry. Offspring weights on L1and 2, head circumference and body length (L2) were measured.Serum of dams and offspring was obtained to determine total T4 andprolactin levels by RIA. Our results show that H mothers had moreimplantation sites and pup number (p