Hyperthyroidism enhances fetal and placental growth and placental immune cell infiltration.

SANCHEZ MB; MORENO-SOSA MT; NEIRA FJ; PIETROBON EO; MACKERN OBERTI JP; SOAJE M; JAHN GA; VALDEZ SR

Mar del Plata

Congreso; LXIV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2019

Sociedad Argentina de Investigación Clínica

Thyroid dysfunctions cause reproductive disorders as fetal deaths, preterm birth and preeclampsia. Whether thyroid hormones (THs) exert any function in placental immune cells is unknown. Therefore, the aim of our work was to assess the influence of hyperthyroidism on placental immune cells as well as the impact on reproduction in pregnant rats. To this end, 10-12 weeks old Wistar rats were injected with a daily dose of T4 (0.1 or 0.25 mg/kg s.c) to induce hyperthyroidism (hyper) or vehicle in control animals. Rats were mated 8 days after starting T4 treatment and euthanized on day 19 (G19) and 20 (G20) of gestation. Placenta samples were minced to reach single cell suspension. Then, resident placental immune cells (CD45+) were analyzed by flow cytometry and mRNA content of hormone receptors by qPCR. Also, placental and fetus weights and fetus number were measured. Hyper mothers delivered more fetuses compared to controls (p