Role of calcium channel TRPV4 in the Acuaporin 2-dependent renal cell migration

RIVERA MF; BELTRAMONE N; RIVAROLA V; FORD P; CAPURRO C; DI GIUSTO G

Virtual

Congreso; Reunion Anual Sociedad Argentina de Fisiolgia; 2021

Sociedad Argentina de Fisiolgia

We have previously shown that Aquaporin 2 (AQP2), in addition to its canonical role as a water channel, promotes renal cellmigration. This promigratory effect is due, at least in part, to the modulation of Na+/H+ exchanger (NHE1) activity. Since NHE1activity is highly modulated by calcium and we showed a physical and functional interaction between AQP2 and calcium channelTRPV4, we propose to further investigate TRPV4 contribution in AQP2-dependent renal cell migration. For experimentalprocedures, we used a renal cell line stably transfected with AQP2: AQP2-RCCD1. We evaluated TRPV4 participation in collectiveand individual cell migration through wound healing and cell tracking assays in presence of the potent specific agonistGSK1016790A (GSK, 10nM) and the selective inhibitor HC‐067047 (HC, 10μM). Cell viability was checked with trypan blue staining.Finally, TRPV4 localization was analyzed with immunofluorescence assays. Our results showed that, both inhibition and activationof TRPV4, reduce collective cell migration (Control: 30.44±0.59%, n=39; HC: 24.72±0.92%, n=20; GSK: 15.65±1.79%, n=9; p