Role of NHE1 and AQP2 in basal and acidosis associated apoptosis in a model of renal cortical collecting tubule principal cells: mpkCCDc14

MAZZOCCHI M; PORTA M; DI GIUSTO G; PIZZONI A; BELTRAMONE N; FORD P; CAPURRO C; RIVAROLA V

Buenos Aires

Congreso; Reunion Sociedades Biocientificas; 2017

Sociedad Argentina de Fisiologia

The association between growth mechanisms and intracellular pH(pHi) elicits the possibility that a chronic pH alteration modifies cellgrowth. Control of pHi is regulated in part by the isoform 1 of Na/Hexchanger (NHE1), an exchanger that, depending on the cell type,is involved in apoptotic or proliferative processes. We previouslyshowed in renal cells that the expression of aquaporin 2 water channel (AQP2) modifies growth. The aim of this study was to investigate whether exposure of renal cells to acid medium altered apoptosis and, if so, its association with NHE1 and AQP2. To investigate this, we used a kidney principal cell line whose AQP2 expression can be induced by arginine vasopressin (AVP): mpkCCDc14. Cell number counting, apoptosis and immunofluorescence studies were performed. Our studies show that after being cultured for 11 days in the presence of AVP mpkCCDc14 cells increase their expression of both AQP2 and NHE1. AVP stimulated cells have a higher basal apoptosis than control ones (% apoptosis control 7.75±1.37 vs AVP 13.33±1.85, p