INVESTIGADORES
PETRILLO Ezequiel
congresos y reuniones científicas
Título:
DECIPHERING THE NATURE OF A RETROGRADE SIGNAL THAT REGULATES ALTERNATIVE SPLICING IN ARABIDOPSIS
Autor/es:
EZEQUIEL PETRILLO; MICAELA A. GODOY HERZ; ALBERTO R. KORNBLIHTT
Reunión:
Congreso; SAIB - XLVI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Resumen:
With the aim of understanding alternative splicing regulation in
plants we analyzed about 100 alternative splicing events with a
high-resolution RT-PCR panel and found that 40% of them respond
to light/dark transitions. We chose the mRNA of the SR protein
Rsp31 as a model to investigate the mechanisms involved. Light
exposure, perceived by the chloroplast, increases the proportion of
the functional mRNA splicing isoform of RSp31. The effect is only
observed in roots that have not been dissected from shoots before
light exposure, suggesting that the signal is able to travel from
leaves to roots. Chloroplast signals include reactive oxygen species
(ROS), sugars, the redox-state of the electronic transport chain,
plastid gene expression and chlorophyll metabolism. Mutants of the
chlorophyll biosynthesis pathway behave as wild type plants, ruling
out the involvement of this pathway. Both H O and sucrose mimic 2 2
the effects of light on Rsp31 alternative splicing. However,
flavodoxin-overexpressants do not inhibit the effect of light, and
methylviologen treated plants behave as control plants, which
favors a role for sugar signaling but does not completely rule out
ROS. To get deeper insights into the nature of the signaling we are
currently using Arabidopsis mutants and drugs that affect the redoxstate
of the plastoquinone pool.