Radiosensitization of human melanoma cells by superparamagnetic iron oxide nanoparticles

GRISSI, C.; TAVERNA PORRO, M.; PERONA, M.; MORENO, S.; SACANELL, J.; DEL GROSSO, M.; IBAÑEZ, I. L.; DURÁN, H.

Congreso; Reunión Conjunta de Sociedades de Biociencias 2021, Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), Reunión Anual de la Sociedad Argentina de Inmunología (SAI), Reunión Anual de la Asociación Argentina de Farmacología Experimental; 2021

SAIC, SAI, AAFE, NANOMED-ar

Melanoma is the deadliest form of skin cancer, highly metastatic and resistant to therapies. Superparamagnetic iron oxide nanoparticles (SPIONs) have shown great potential for diagnosis and therapy. The aim of this study was to evaluate the radiosensitizing effect of SPIONs in A375 human melanoma cells. SPIONs were synthesized and stabilized by methyl-poly(ethyleneglycol). After that, it was physicochemically characterized by transmission electron microscopy, X-ray diffraction, magnetometry and tested in vitro. Superparamagnetic behavior and low dispersion in shape and sizes (8-17 nm) were obtained. No cytotoxicity was found in A375 cells exposed up to 250 µg/ml for 24 h. Dichloro-dihydro-fluorescein diacetate assay revealed higher reactive oxygen species levels in treated cells (p