Effects of gallic acid on myocardial ischemia-reperfusion injury: involved mechanisms

GONZÁLEZ ARBELÁEZ LF; SCHINELLA GR; MOSCA SM

La Plata

Congreso; Reunión anual Sociedad Argentina de Fisiología SAFIS 2021; 2021

Introduction: It was previously demonstrated that gallic acid, a natural phenolic compound found in several fruits and medicinal plants, have several health-promoting effects. Objectives: To determine the effects of gallic acid on ischemia-reperfusion injury analyzing the involved mechanisms.Methods: Isolated rat hearts perfused by Langendorff system were assigned to the following groups:1)Non ischemic control (NIC):110 min of perfusion; Ischemic control (IC): 30 min of normothermic global ischemia and 60 min of reperfusion (R); GA group: 0.25, 0.5 and 1µM gallic acid was administered during the first 10 min of R. Infarct size (IS) was determined by TTC staining. Systolic and diastolic function were assessed by left ventricular developed pressure (LVDP) and left ventricular end diastolic pressure (LVEDP), respectively. Left ventricle of other hearts (n=3), submitted to the same protocols, were used to determine the expression of phosphorylated forms of Akt, eNOS, GSK-3 and PKCε by western blot. Data are given as means ± SE and analyzed using repeated measures of two-way analysis of variance (ANOVA) with Turkey´s post-test for multiple comparisons among groups. A p value