CHARACTERIZATION OF AHCYL1 AND KI67 EXPRESSION IN NON-SMALL-CELL LUNG CANCER

MELINA MUÑOZ BERNART *AUTHORS CONTRIBUTED EQUALLY; ARACELI CASTRO, *AUTHORS CONTRIBUTED EQUALLY; NICOLAS BUDNIK; SEBASTIÁN DEFRANCHI ; GUSTAVO PARRILLA ; JUAN PABLO SANTILLI ; KEVIN DAVIES; LAURA THOMAS; CARLOS VIGLIANO , # CORRESPONDENDING AUTHORS; CAROLINA PEREZ CASTRO, # CORRESPONDENDING AUTHORS

Capital Federal

Congreso; SAIC 2021; 2021

CHARACTERIZATION OF AHCYL1 AND KI67 EXPRESSION IN NON-SMALL-CELL LUNGCANCERMelina Muñoz Bernart 1* , Araceli Castro 2 *, Nicolás Budnik 1 , Sebastián Defranchi 2 , GustavoParrilla 2 , Juan Pablo Santilli 2 , Kevin Davies 2 , Laura Thomas 1 , Carlos Vigliano 2# , CarolinaPerez Castro 1#1 Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA) - CONICET - Partner Institute of the Max Planck Society.Buenos Aires, Argentina.2 Hospital Universitario Fundación Favaloro - Instituto de Medicina Traslacional, Trasplante y Bioingeniería (IMeTTyB) -Universidad Favaloro ? CONICET, Buenos Aires, Argentina*Authors contributed equally# Correspondending authorsLung cancer is an extremely malignant disease due to its metastasis andrecurrence. In particular, non-small cell lung cancer (NSCLC) is characterized byhigh cellular and molecular heterogeneity. Recent research has reported thatAHCYL1 (adenosylhomocysteinase-like 1) protein could be involved in theregulation of autophagy and tumor cell proliferation processes. We havepreviously shown that AHCYL1-depleted cells have higher tumorigenic capacity;however, the expression and the potential role of AHCYL1 in lung cancer remainunclear. We explored the expression of AHCYL1 and Ki67 (a cell proliferationmarker and indicator for poor prognosis in many cancers) in patients with NSCLCby immunohistochemical analysis; along with transcriptomic data analysis ofpublic databases. RNA-seq data revealed that AHCYL1 is expressed in all stages oflung cancer, and notably, it shows an inverse correlation with the expression ofstemness-related genes. Proliferation marker Ki67 also showed an inversecorrelation with AHCYL1 (p