Conferencia: Ceramide and the FasR in plasma membrane spheres

CARRER, D. C.; WEIDEMANN, T; RIES, J.; SCHWILLE, P.

Boston

Congreso; 53va Annual Meeting de la Biophysical Society; 2009

Biophysical Society

We have induced plasma membrane spheres formation in human cells in culture expressing a FasR-eGFP construct in order to study the effect of the membrane lipids organization in the receptor lateral distribution, diffusion and aggregation state. The induction of domain coalescence with Cholera Toxin subunit B produced micron-sized domains into which the receptor preferentially partitioned. We have used fluorescence correlation spectroscopy to assess the effects of domain coalescence, FasL binding, an agonistic antibody and sphingomyelinase treatment on the dynamics of lipid and receptor molecules in the membrane.