Evaluation of an Immunoagglutination Assay for the Diagnosis of Trypanosoma Cruzi Infection

GARCÍA, V.; GONZALEZ, V.D.; VEGA, J.R.; MARCIPAR, I.; GUGLIOTTA, L.M.

Heredia

Simposio; XII Simposio Latinoamericano de Polímeros SLAP 2010 - X Congreso Iberoamericano de Polímeros; 2010

Biomolecules are attached onto latex particles either by simple physical adsorption (PA) or by covalent coupling (CC). The latex-protein complexes obtained by PA have low stability and can give unspecified agglutinations. Moreover, the complexes are considered of low quality, due to the potential desorption and denaturalization of the adsorbed proteins. For the chemical coupling of proteins, hydrophilic functionalized latexes of uniform particle size are used. Their hydrophilic nature increases the stability of the latex-protein complexes, and it also prevents nonspecific latex-protein interactions. In this work, the sensitization and application results of latex-protein complexes for detecting anti-T. cruzi IgG in human sera are presented.