Hydatid fluid from echinococcus granulosus induce the autophagy process in dendritic cells and promote antigen presentation and T- cell proliferation.

CHOP, MAIA; PLA, NATALIA; LOOS JULIA .A.; NICOLAO MA. CELESTE; CUMINO ANDREA C; RODRIGUEZ R CHRISTIAN

Buenos Aires

Congreso; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica Reunión Anual de Sociedades de Biociencias; 2020

Sociedad Argentina de Investigaciones Clínica y Sociedades Conjuntas

Background: Autophagy is an important process for the presentationof endogenous and exogenous proteins on MHC I and II molecules,promoting activation of CD8+ and CD4+ T cells respectively. Theaim of this work is to analyze if hydatid fluid (HF) from Echinococcus,constituted by a wide range of parasite proteins could trigger autophagyimproving antigen presentation and T cell proliferation. Methods:BMDCs were cultured in complete RPMI. Hydatid fluid (HF) waspunctured from the hydatid cysts collected of infected cattle slaughtered.Antigen uptake was measured with (FITC-OVA) in BMDCsusing a standard method. HF-stimulated BMDCs, were evaluated inautophagy induction and MHC II expression. For it, fixed cells wereimmunostained with LC3-b (clone H50) and analyzed them by immunofluorescenceconfocal microscopy. CFSE-stained splenocyteswere co-incubated with BMDCs using a DC: splenocyte ratio of 1:4Cellular proliferation was assayed after 4 days of culture by flowcytometry. Results: First, we evaluated if stimulation of HF during 18h in BMDCs, induce different rates of antigen uptake. Effectively, thepresence of Echinococcus antigens induces a markedly decreasedOVA-uptake compared to control (**p