INVESTIGADORES
ALVAREZ Hector Manuel
congresos y reuniones científicas
Título:
Extracellular lipids produced by different Rhodococcus species
Autor/es:
VILLALBA MS; ALVAREZ, HÉCTOR M
Lugar:
PUERTO MADRYN
Reunión:
Congreso; XLVI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2010
Institución organizadora:
SAIB
Resumen:
Rhodococcus bacteria
accumulate variable amounts of triglycerides (TAG) during cultivation
on diverse substrates. The intracellular TAG accumulation by rhodococci
is a well established feature, but the extracellular export of TAG
remains to be investigated. In this study we analyzed the ability of Rhodococcus species, such as R.opacus, R.jostii, R.wratislaviensis, R.ruber, R.erythropolis and R.fascians,
to produce extracellular TAG. All studied strains were able to export
TAG into the culture medium, and in proportion, those species with
lower content of intracellular TAG, produced higher amounts of
extracellular TAG. Interestingly, intracellular and extracellular TAG
produced by all studied species exhibited different fatty acid (FA)
composition as revealed by preparative thin layer and gas
chromatography analyses. Monounsaturated FA occurred predominantly in
intracellular TAG, whereas extracellular TAG contained almost
exclusively saturated FA (86.5-100 % of total FA). Extracellular TAG
from all rhodococcal species were composed predominantly by C16:0 and
C18:0 (76-93 %), while C18:1 was one of the major FA in intracellular
TAG. The presence of TAG with different FA composition inside and
outside the cells suggests the occurrence of an export mechanism for
lipids in rhodococci which probably involve export proteins.