INVESTIGADORES
ABRAHAM Gustavo Abel
congresos y reuniones científicas
Título:
Highly-porous electrosprayed scaffolds of compatibilized PCL/PDIPF blends for bone tissue engineering
Autor/es:
J.M. FERNÁNDEZ; G.A. ABRAHAM; M.S. CORTIZO
Lugar:
Rosario, Santa Fé
Reunión:
Taller; 1º Taller de Órganos Artificiales, Biomateriales e Ingeniería de Tejidos (BIOOMAT); 2009
Institución organizadora:
UNR, CAIC y SLABO
Resumen:
Synthetic biodegradable
polyesters are being extensively studied as biocompatible polymeric
scaffolds for different tissue engineering applications. Poly(e-caprolactone) (PCL) and poly(diisopropylfumarate) (PDIPF) have probed to support adhesion,
growth and differentiation of two osteoblastic cell lines, mouse
calvaria-derived MC3T3E1 cells and UMR106 rat osteosarcoma, suggesting
that these polymers could be useful in bone tissue regeneration. The
blend of these polymers can modulate the biodegradation rate. The
compatibility between PCL and PDIPF can be improved by ultrasound. For
these reason solutions of PCL/PDIPF blends were exposed to high-intensity ultrasound. Size
exclusion chromatography demonstrated that chain scission produced
macroradical formation. Moreover, sonochemically induced reactions led
to interpolymer radical coupling and block copolymer formation, which
acted as compatibilizer between PCL and PDIPF. The compatibilized
PCL/PDIPF blend had better properties than the non-sonicated one. In
this work, highly-porous scaffolds of the compatibilized blend were
prepared by electrospinning. A polymer solution 4% wt/V in chloroform
was delivered through a spinneret at a flow rate of 1.5 mL/h, using an
applied voltage of 0.7 kV.cm-1. The sample was collected on
an aluminum foil covering the grounded aluminum collector. Experiments
were performed during 1 h. The solution properties (solvent,
concentration) and the processing conditions used in this work allowed
the deposition of wet microspheres, which aggregated on contact with
each other, instead of micro/nanofibers. The electrosprying generated a
highly-porous interconnected structure formed by agglomerated
microspheres. The obtained microspheres were irregularly shaped with
approximately 5 mm
in diameter and a narrow size distribution, as observed by scanning
electron microscopy. Thus, the electrospraying technique could be a
promising technique for the preparation of PCL/PDIPF scaffolds for bone
tissue engineering. Additional experiments, varying solution
concentration and processing parameters, could generate scaffolds with
micro/nanofibrous morphology.