Estudio in vivo de la interacción de la proteína p0 supresora del silenciamiento del potato leafroll virus (plrv-ar) con proteínas del hospedador.

BARRIOS BARÓN, MARÍA PILAR; DELFOSSE, VERÓNICA C.; NAHIRÑAK, VANESA; ALMASIA, NATALIA INÉS; AGROFOGLIO, YAMILA C.; VAZQUEZ ROVERE, CECILIA; DISTÉFANO, ANA J.

Cordoba

Congreso; XXXII Reunión Argentina- XVI Congreso Latinoamericano de Fisiología Vegetal (RAFV 2018); 2018

Potato leafroll virus (PLRV) is one of the most damaging potato viruses and causes significant economic losses. This virus produces animportant disease within all major potato cultivated regions. PLRV 5?-proximal ORF, ORF0, encodes P0 protein which is involved in symptomdevelopment and acts as a silencing suppressor protein. P0 proteins encoded by polerovirus members were shown to induce the degradationof the RNAi surveillance protein ARGONAUTE1. P0 mechanism of action depends on the interaction through an F-box like domain withmembers of the host SKP1-Cullin-F-box (SCF) family of E3-ubiquitin-ligases. Since this interaction varies among the different PLRV isolates,we study the in vivo interaction between P0 of the argentinian PLRV isolate (P0PL-Ar) and SKP1-like proteins of Arabidopsis (ASK1 andASK2) and potato (StSKP1). We also analyze whether the conservation of P0 F-box domain is not only necessary for such interaction butalso for the maintenance of its suppressor activity. We analyzed the interactions between wild type or mutated P0 versions and SKP1 proteinsby yeast two-hybrid system. For the first time, we demonstrated that P0PL-Ar interacts with ASK1, ASK2 and StSKP1. In addition, theseinteractions, and P0PL-Ar suppressor activity are abolished by substitutions of essential residues in the F-box-domain