Commercial vaccine against bovine herpervirus 1: humoral and cellular immune response, viral protection and endosomal toll-like receptor expression

CHEUQUEPÁN VALENZUELA F; KORNUTA C; BIDART J; SORIA I; GAMMELLA M; ODEÓN A; QUATTROCCHI V; HECKER Y; MOORE D; ROMERA SA; ZAMORANO P; MARIN M; LANGELLOTTI C

Online

Simposio; Innate Immunity: Mechanisms and Modulation.; 2021

Keystone Symposia on Molecular and Cellular Biology

Toll-like receptors (TLRs) are a component of innate immunity. The TLR expression is highly regulated by microbial molecular patterns, and could be a target for the development of an improved immune response, desirable aspects with the use of vaccines. The TLR expression after immunization of bovines against bovine herpesvirus 1 (BoHV-1) has not been characterized. The aim of this work was to evaluate the gene expression of the endosomal TLRs in peripheral blood mononuclear cells (PBMC) from calves previously immunized with an inactivated vaccine against BoHV-1. Seronegative bovines to BoHV-1 were subcutaneously vaccinated with Biopoligen Air (Biogenesis-Bagó, Argentina) Commercial Vaccine (n=4) at 0 and 30 days or a negative control group (n=4) remained unvaccinated. At 90 days post-vaccination (dpv) the animals were challenged with BoHV-1 LA strain (Quattrocchi et al., 2017). Total RNA was extracted from PBMC obtained at 33 dpv (3 days post-booster) to determinate the gene expression of TLR3, TLR7-TLR9 by RT-qPCR (Marin et al., 2016). Relative expression analysis was performed with REST software (p