Use of endogenous lipids as unique oxidative substrates during the in vitro maturation of porcine oocytes

FLORENCIA IRIARTE; PABLO CETICA; CAROLINA LETO; GIULIANA TRICERRI; SERGIO MORADO; FRANCO PORTILLO; ELIZABETH BREININGER

Mendoza

Congreso; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; 2020

SBCu, SBC, ABT, SBR, SAB, SCHRD

It has been postulated that endogenous lipids can be used as energy substrates during the in vitro maturation of porcine oocytes. However, those studies were conducted in complex culture media with various substrates, so the use of each metabolite could be overlapped. The main aim of this work was to analyze the implication of the metabolism of endogenous lipids as unique oxidative substrates during porcine oocyte in vitro maturation. Ovaries from slaughtered gilts were transported in an isothermal solution to the laboratory and subsequently prepared for follicle aspiration. Immature cumulus-oocyte complexes (COCs) were obtained by the aspiration of antral follicles (3-8 mm) and were morphologically classified by means of stereomicroscopes according to the characteristics of their cumulus. Only those oocytes surrounded by a dense and integral cumulus were selected and matured in the following culture media: a) NCSU-37 (without pyruvate and glucose); b) NCSU-37 + glucose (as positive control); c) NCSU-37 + L-carnitine (to stimulate β-oxidation); d) NCSU-37 + glucosa + L-carnitine and e) NCSU-37 + etomoxir (to inhibit β-oxidation). The percentages of nuclear maturation were evaluated using an epifluorescence microscope by the presence of metaphase II after incubating the oocytes with Hoechst 33342 stain. The use of endogenous lipids was determined using Nile Red stain. Values with p