Aortic large-conductance calcium activated potassium channel and potassium channel with inwardly rectification are differently expressed in normotensive and hypertensive rats.

SOSA AGUSTINA; SORIA DAMIÁN; RAMÍREZ HERNÁN; TOMAT ANALÍA; ELESGARAY ROSANA; ARRANZ CRISTINA; CANIFFI CAROLINA

Congreso; Reunión de Sociedades de Biociencias 2020, LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC) LXVIII Reunión Anual de la Sociedad Argentina de Inmunología (SAI) Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS); 2020

Potassium channels and C-type natriuretic peptide (CNP) play key roles in the regulation of vascular tone. Objective: to evaluate the aortic expression of BKCa and Kir channels in spontaneously hypertensive rats (SHR) and their role in the vasodilator effect of CNP.Experimental design: Male Wistar rats and SHR (14 weeks-old) were sacrificed and aorta was removed to determine: BKCa and Kir2.0 protein expression and vascular reactivity after CNP addition (1pmol/L - 1umol/L). Denuded aortic rings were pre-contracted with phenylephrine (1umol/L) in the presence or absence of tetraethylammonium (TEA, non-selectively potassium channel blocker, 1mmol/L), iberiotoxin (IbTx, BKCa blocker, 30nmol/L), or BaCl2 (Kir blocker, 30 umol/L). Statistical analysis: Results are expressed as mean±SEM, Student t-test or 2-way ANOVA, followed by Bonferroni posthoc test; n=4-6/group.Results: Protein content of BKCa and Kir2.0 was lower in SHR compared to Wistar rats (BKCa: Wistar=1.1±0.1 vs. SHR=0.7±0.1*; Kir2.0: Wistar=0.8±0.1 vs. 0.4±0.1*; *p