DETERMINATION OF PHENOL, ANTHOCYANIN, FLAVONOID, AND TANNIN CONTENT AND EVALUATION OF THEIR ANTIOXIDANT ACTIVITY IN Chenopodium quinoa EXTRACTS

CARLA ANTONELLA BURGOS

SAN MIGUEL DE TUCUMAN

Jornada; XXXV JORNADAS DE BIOLOGIA; 2021

Chenopodium quinoa, a species cultivated by the Andean peoples of America for thousands of years,is a product of great cultural value that also has a high nutritional value. Its fruit is an achene containing only oneseed of variable coloration, with a diameter of 1.5 to 4mm.Objective: To quantify phenols, anthocyanins, flavonoids, tannins and determine their antioxidant capacity inextracts of Chenopodium quinoa seeds.Materials and Methods: The seeds come from Tafí del Valle, Tucumán. In 2 seeds extracts (I, hydro-alcoholic andII, acetonic), we quantified phenols, anthocyanins, flavonoids, tannins and determined their antioxidant capacity byFRAP, purification of the ABTS radical and reduction of molybdenum.Results: Quantifications showed: Phenols in I: 771.30mg EAG/100g e.s.; II: 793.80mg EAG/100g e.s. AnthocyaninsI: 28.50mg EM3Glu/100g e.s.; II: 22.60mg EM3Glu/100g e.s. Flavonoids in I: 173.80 mg EQ/100g e.s.; II: 91.70 mgEM3Glu/100g e.s. Tannins in I: 341.40mg EC/100g e.s.; II: 406.30mg EC/100g e.s. Antioxidant Capacity: FRAP inI: 278.10mg AA/100g e.s.; II: 182.80mg AA/100g e.s. Depuration of the ABTS radical in I: 97.86 IC50 (μg/mL), II:72.90 IC50 (μg/mL). Molybdenum reduction Mo+6 to Mo+5 in I: 92.50mg EAA/100g e.s., II: 142.40mg EAA/100g e.s.Conclusions: The analyses allowed us to determine that extract I, hydro-alcoholic Chenopodium quinoa, has amoderate antioxidant capacity greater than extract II, acetonic, showing potential for use with nutritional and / orpharmaceutical uses.