Protein nanoparticles as carriers of natural antioxidants

SOFIA L. CANDIDO; FERNANDO C. ALVIRA; JUAN C. MORENO; LUIS MARTINEZ; SILVIA DEL V. ALONSO

San Luis

Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Biofísica; 2019

Sociedad Argentina de Biofisica y Universidad Nacional de San Luis

We have conducted physically and rheological characterization of a protein nanocarrier(bovine serum albumin nanoparticles) to encapsulate antioxidants of natural origincoming from blueberries (Vaccinium Myrtillus). The blueberries are rich in anthocyanin(A). The encapsulation was done with the aim of obtaining a stable formulation of NP BSA+ A that can be useful to enrich food or to be injected into the body. Due to BSA NP isformed by an unknown number of individual BSA molecules, computational simulationswere performed to estimate this number.BSA NPs are obtained by a chemical method and stabilized by Gamma irradiation.Antioxidants are obtained by liquid-solid extraction from blueberries. Thenanoformulation was studied by, Dynamic Light Scattering (DLS), potential Z, electrontransmission microscopy, rheology, UV-VIS and fluorescence spectroscopy at threedifferent temperatures, 8 °C (storage temperature), 25 °C (environment temperature),37 °C (physiological).Our results show that the BSA NPs obtained by elution in a molecular exclusion columnhas a hydrodynamic radius of 28 +/- 5 nm and is composed of approximately 183 BSAmolecules. Depending strongly on the temperature.It was possible to study the interaction of NP BSA + A and discriminate the active sitewhere it occurs (Sudlow I and/or II), by fluorescence. From the rheological tests, it wasobserved that for all three temperatures the samples tend to a behave as Newtoniantype and that there is a correlation between viscosity and temperature values. Themorphological changes observed by TEM corroborate the relationship of viscosity valueswith aggregate formation.