Leptin promotes trophoblastic cell invasion involving Catenin/Wnt pathway

TAMARA FERNÁNDEZ ARANGUREN; RIEDEL, RODRIGO; MALENA SCHANTON; AYELÉN TORO; MARIANA JAIME; BERNARDO MASKIN; JULIETA L. MAYMÓ; CECILIA L. VARONE

Congreso; LXIII Reunión de la Sociedad Argentina de Investigación Clínica; 2018

Leptin promotes trophoblastic cell invasion involving Catenin/Wnt pathway Tamara Fernández Aranguren1, Rodrigo Riedel1, Malena Schanton1, Ayelén Toro1, Mariana Jaime2, Bernardo Maskin2, Julieta Maymó1 and Cecilia Varone11 Departamento de Química Biológica FCEN-UBA. Instituto de Química Biológica FCEN, IQUIBICEN, CONICET2 Hospital Nacional Profesor Alejandro PosadasLeptin, the adipocyte derived hormone encoded by the Lep gene, is synthesized by placental trophoblasts where it works as a paracrine and autocrine hormone. It has been shown that during pregnancy leptin modulates migration and invasion of trophoblastic cells. In recent studies it has been determined that leptin reduces the level of E-Cadherin and increases the expression of 1-Integrin in Swan-71 cells.In the present work, we aimed to study the molecular mechanisms that mediate the effect of leptin regulation of trophoblastic cells invasion in placenta.We use Swan-71 cells, a first trimester trophoblastic human cell line, cultured under standard conditions, as well as human term placenta explants. Swan-71 cells and placental explants were treated with different concentrations (50, 100 and 250 ng/ml) of recombinant leptin during 48 h (Swan-71cells) or 20 h (placenta explants). Metalloproteinases 2 and 9 (MMP-2 and MMP9) were assessed by qRT-PCR and zymography. -Catenin expression was determined by Western-Blot and immunofluorescence analysis. Invasion experiments using trans-wells covered with Matrigel were also performed.We determined that leptin treatment increased invasion of trophoblastic cells. Leptin also enhanced MMP-2 and MMP-9 expression and activity. The effect of leptin on the expression of -Catenin as a possible activated pathway mediating invasion was studied. We determined that leptin treatment increased significantly the level of -Catenin in Swan-71 cells (p