THE UV FILTER BENZOPHENONE 3 ALTERS BLASTOCYSTS IMPLANTATION AND THE EARLY EMBRYONIC DEVELOPMENT

ABUD, J.E.; SANTAMARÍA, CG; ZENCLUSSEN ML; RODRÍGUEZ HORACIO ADOLFO

Mar del Plata

Congreso; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2020

SAIC

The aim of our study is to analyze the effect of the UV-filter benzophenone3 (BP3) on early gestational processes by a complementaryin vitro and in vivo approach. Using an in vitro assay of blastocystimplantation, we analyzed the effect of three different BP3 concentrations:a) BP3-2: the predicted non-effect concentration (PNEC: 2μg/L), b) BP3-20: the concentration detected in the amniotic fluid (20μg/L) in our previous studies and c) BP3-200: the plasma concentrationsreported in humans (200 μg/L). Blastocysts from 3.5 dayspregnant mice (C57BL/6J) were transferred to a monolayer of autologousuterine epithelial cells (UECs) and cultured in the presenceof vehicle (0.01 % DMSO) or BP3 for 6 days. Blastocyst expansion,hatching and implantation in the monolayer as well as implantationarea were analyzed microscopically and recorded every 12 h. Toverify the in vivo relevance of the in vitro results, pregnant C57BL/6Jmice were exposed via dermal route to BP3-50 (50-mg BP3 kg.day)or olive oil (vehicle) from gestation day (gd) 0 to gd10. The micewere sacrificed at gd10 and the size of the whole implantation sites(WIS) was measured.In vitro exposure to BP3-2 and BP3-200 altered the blastocystsexpansion. Moreover, the hatching and the implantation time weredelayed and the implantation areas were significantly smaller thanthose from the control with all BP3 concentrations assayed. Invivo study reaffirms the in vitro results, since we found that BP3-50-exposed WIS was smaller than those exposed to the vehicle.Previously, we showed that in vivo dermal BP3-50 produced an intrauterinegrowth restriction (IUGR) phenotype and lower offspringweight of first progeny. Here, we could demonstrate that BP3 disruptsblastocyst implantation and early embryo development. Ourresults suggest that an underlying mechanism by which BP3 affectspregnancy are linked to disruption of the implantation stage, leadingconsequently to a reduction in embryo size.