Neuroprotective effects of 17b-Estradiol administration on dopaminergic synthesis in an animal model of Parkinson disease

MP BONACCORSO MARINELLI; S GÓMEZ; S VALDÉZ; R CABRERA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias; 2019

SAIC-SAFE- SAB-SAP-AACYTAL-NANOMED-ar-HCS

We investigate estrogen neuroprotective actions in adult male rats brain tissue after neurotoxic injury. Using inmunohistochemical techniques (IHQ), we label and localize neurons that experess tyrosine hydroxylase (TH) on substantia nigra (SN) and their projections to corpus striatum (CPu). TH is the first enzyme in dopamine (DA) biosynthesis and catalyses the conversion of L-tyrosine to L-DOPA. We use it to quantify cell loss in SN and analyze striatal dopamine depletions.On postnatal day 60 (D=0) rats were injected with 6-hydroxydopamine (6-OHDA) or vehicle (V) in the left CPu. From D 7-17, they received a chronic (10 days) treatment with 17b Estradiol (E= 0.1 μg/kg/day s.c) or corn oil. Groups were conformed as HP (6-OHDA lesion, O n=18); HP+E (6-OHDA lesión, E treatment n=18); E (V lesion, E treatment n=15); C (V lesion, O treatment n=15). On D 60 all animals were euthanized for TH IHQ.In SN, the number of TH+ cells (NN) on both hemispheres was statistically lower in HP animals in comparison to the other groups, p