Ultrastructure of the adhesion of Pseudobutyrivibrio xylanivorans to cancer cells

GRILLI, D; GIMENEZ, MC; MANSILLA, ME; PÁEZ LAMA, S.; ARENAS, GN; TELECHEA, A; TEREBIZNIK, M

San Luis

Jornada; XXXVII Reunión científica anual de la Sociedad de Biología de Cuyo.; 2020

Sociedad de Biología de Cuyo

Pseudobutyrivibrio xylanivorans (Px), a wild-type bacterium isolated from rumen of Creole goats, has been intensively studied during this decade. Px strains generate an acidic environment through the production of butyrate and lactate. In addition, Px produce bacteriocins that inhibit some potential pathogenic bacteria and produce the biohydrogenation of linoleic acid. Butyrate, an important nutrient for normal colonic cells, reduces proliferation and induces apoptosis of human colorectal adenocarcinoma (HCRA) cells. Bacterial adhesion to the epithelium of the digestive tract of the host is a fundamental requirement for the selection of probiotics, since it influences the time of permanence of bacteria in gut. Recently, we have shown that Px exhibits significantly greater adherence to HCRA cells, compared to demonstrated adherence to He-La cells, a non-colonic epithelial cell line. In order to deepen the knowledge of the mechanism of adhesion of Px, we use scanning electron microscopy (SEM) to examine adhesion to SW480 cells (Dukes type B colorectal adenocarcinoma cells). SEM micrographs showed that 1 h after interaction. Px was trapped on the surface of the cancer cell, on laminar structures surrounding discrete segments of the bacterium, as described above for the membrane wraps (MWs). For all the above, we consider to deepen the study of the molecular mechanisms that underlie the phenomenon of adhesion of Px to HCRA cells.