METABOLIC ALTERATIONS IN AMYGDALA INDUCED BY THE ACUTE ADMINISTRATION OF KETAMINA (Preliminary study)

GUEVARA MANUEL ALEJANDRO; LORENZO ACQUARO SOFÍA; CUNIETTI MARTIN JOSEFINA; GARCÍA MENENDEZ SEBASTIÁN; LAFUENTE JOSÉ VICENTE; BAIARDI GUSTAVO; GARGIULO PASCUAL ÁNGEL

Mendoza

Simposio; II simposio internacional de medicina traslacional 2019 XIV Jornadas de inVestigacion FCM UNCUyo; 2019

Las Facultades de Ciencias Médicas y de Ciencias Exactas y Naturales de la UNCuyo, junto a la Facultad de Farmacia y Bioquímica de la UBA y la Facultad de Medicina de la Albert-Ludwigs-Universität de Freiburg, Alemania

Introduction and objectives: Ketamine is a general anaesthetic that acts as an antagonist of the N-methyl-D-aspartic acid (NMDA) receptors, as well as the psychotrogenic compound Phencyclidine (PCP), a drug recognized for its drug-induced psychotogenic effect. In subanesthetic doses, ketamine produces hallucinations, and for this reason it has been used as a model of schizophrenia in animals. The behavioral disorders induced by this compound in subanesthetic doses are subject of study in our laboratory. Materials and methods: In the present work we studied the effect produced by the systemic administration of ketamine at subanesthetic doses on metabolic activity in amygdala in male rats derived from the Holtzman strain (240-280 g). Three doses were systemically administered in a saline vehicle. They were compared: 1.25, 2.50 and 5 mg / kg (n = 5 per group). The metabolic activity was evaluated using MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) through the spectrophotometric measurement of the wavelength. Results: Metabolic activity showed a clear and significant decrease in the amygdala (1.25 mg / kg, p