KETAMINE DISRUPTS WORKING MEMORY IN A HOLE BOARD TEST AND THE METABOLIC ACTIVITY IN PREFRONTAL CORTEX AND NUCLEUS ACCUMBENS

GUEVARA MANUEL ALEJANDRO; MARQUEZ HERRERO SANTIAGO NICOLÁS; GARCÍA MENENDEZ SEBASTIÁN; GARGIULO PASCUAL ÁNGEL

Mendoza

Jornada; XXXIV Annual Scientific Meeting of the Cuyo Biology Society; 2016

Sociedad de Biología de Cuyo

Ketamine, a N-methyl-D-Aspartic-acid (NMDA) receptor antagonist, is an anesthetic that has psychotogenic effects, and it led to use it inducing animal models of schizophrenia. In the present study the effects of systemic administration ketamine (1; 2,5; 2,5; 5; 10; 15 y 20 mg/Kg ip) were evaluated in a hole board test (HBT). Besides the metabolic activity of the Pre-Frontal Cortex (PFC) and the Nucleus Accumbens Septi (NAS) were simultaneously evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) through wavelength measurement using a spectrophotometer. Male rats derived from a Holtzman colony weighing 240-290 g were used. Hole exploration strategies and locomotor activity were significantly modified in the HBT. Non ambulatory movements were significantly decreased at the dose of 10 mg/kg (p