Acute cerebral behavioral and metabolic ketamine (N-methyl-D-aspartate receptor antagonist) in rats intraperitoneally in passive avoidance test). Partial results.

LORENZO ACQUARO SOFÍA; CUNIETTI MARTIN JOSEFINA; GARGIULO PASCUAL ÁNGEL; GUEVARA MANUEL ALEJANDRO

Mendoza

Simposio; II simposio internacional de medicina traslacional 2019 XIV Jornadas de infestigacion FCM UNCUyo; 2019

Las Facultades de Ciencias Médicas y de Ciencias Exactas y Naturales de la UNCuyo, junto a la Facultad de Farmacia y Bioquímica de la UBA y la Facultad de Medicina de la Albert-Ludwigs-Universität de Freiburg, Alemania

Introduction: Phencyclidine (antagonist of the N-methyl-D-aspartic acid (NMDA) receptors), induces a schizophrenic-like psychosis in humans. Ketamine is a general anesthetic, NMDA antagonist, which at subanesthetic doses induces psychotiform manifestations. We study experimentally it in rats in our laboratory. Hypothesis: Ketamine subanesthetic doses may induce brain metabolic and behavioarl disturbances in rats that could be considered animal models of psychosis. Objectives: The objective of present study is to verify metabolic alterations in prefrontal cortex and hippocampus, by ketamine effect and to correlate those doses with alterations in cognition (passive avoidance). Methods: Passive avoidance test Holtzman male rats (240-280 g) in two groups (n=20): Saline and Ketamine controls (2.5 mg / kg), injected 8 min before the test to interfere with the acquisition. The metabolic activity was measured by the MTT test (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) through the spectrophotometric measurement of the wavelength. Two groups were used: Saline Control (n = 5) and Ketamine (2.5 mg / kg, n = 5). The brain structures to be studied were removed (hippocampus and prefrontal cortex) 8 minutes after the drug or saline was applied. Results: We observed a shorter latency time 1 (p