Evaluation of cell behavior on modified polypropylene with swift heavy ion irradiation

ARBEITMAN C.R.; IBAÑEZ, IRENE L.; GARCÍA BERMÚDEZ G.; DURÁN H.; DEL GROSSO M.; SALGUERO, N.; MAZZEI, R.

Itapema, SC - Brazil

Conferencia; 20th International Conference on Ion Beam Analysis; 2011

Ion Implantation Laboratory, UFRGS, Porto Alegre - Brazil and International Atomic Energy Agency (IAEA)

The cellular activities on the surface of synthetic polymers are a key factor in many biomedical and biotechnological applications. It has been know that certain surface characteristics of the polymer materials such as surface energy, hydrophilicity/hydrophobicity, charge property,topography and chemical composition induces changes in the cellular responses. Several studies have been focused on modifying the surface chemistry to improve the biocompatibility of substrates and to enhance cell attachment and growth [1]. The main characteristic of ion beamirradiation is to induce bio and citocompatibility in controlled conditions and in selected areas of the polymer surface [2].Polypropylene (PP) is a biomaterial that has been widely used as non absorbable surgical suture. However his lack of cell recognition sites and hydrophobic surface leads to poor cell adhesion and spreading which limit their used as culture substrate. We previously reported thatchanges in irradiated PP polymer affect on the initial adhesion of NIH 3T3 fibroblasts [3]. The enhancement of cell adhesion does not mean that the surface is adequate as a substrate for functional cells. The purpose of the present work is to continue the study in order to determine proliferation and changes in cytoskeleton and cell morphology on substrates as a function of different irradiations parameters. We irradiated with sulfur (S) ion-beam provided by the Tandar Accelerator (Buenos Aires, Argentina) at energies of 110 MeV with fluences between 1 x 106 to 2 x 1010 ions cm-2. The modifications of the irradiated surface were evaluated with different techniques. Mouse embryonic fibroblasts NIH 3T3 cell line were cultured on each sample. Cell morphology was observed using phase contrast microscopy and focal adhesion and cytoskeleton proteins with  fluorescence microscopy. Cell shape and inmunocytochemical analysis showed large differences as a function of irradiations conditions. This  results strongly suggests that the underlying substratum induce distinct types of cytoskeleton reorganization and therefore influence cellular  responses.[1] Lee JH, Lee JW, Khang G, Lee HB Jung. Interaction of cells on chargeable functional group gradient surfaces. Biomaterials 1997; 18:351-8.[2] Bačáková L, Mareš V, Bottone M-G, Pellicciari C, Lisá V, Švorčík V. Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture. J.Biomedical Mater Res 2000; 49:369-79.[3] C.R. Arbeitman, M.F. del Grosso, I. Ibañez, G. García Bermúdez, H. Durán, V.C. Chappa, R. Mazzei, M. Behar. Irradiation of Polystyrene and Polypropylene to study NIH 3T3 fibroblasts adhesion. Publicado en Nucl.Instrum. Meth. Phys. Res. B 2010; 268-19: 3059-62.