Inhibition of oxidative stress enzyme activities and genotoxicity of the insecticide imidacloprid in the freshwater fish Autraloheros facetus.

ITURBURU, GASTÓN; PANZERI, ANA; CRUPKIN, ANDREA; MENONE, MIRTA LUJÁN

Congreso; 6th SETAC World Congress 2012; 2012

Imidacloprid (IMI) is a neonicotinoid insecticide with neurotoxic action that is at the moment one of the insecticide with the world’s fastest growing sales. In this study, biochemical and genetic parameters of the freshwater fish Australoheros facetus after acute exposure to IMI are presented. Glutathione-S-transferases (GST) and catalase (CAT) activities, malondialdehide content (MDA) in liver and gills and micronucleus frequency (MN) in blood were analyzed. Fish were exposed to: 0 (negative control), 1, 10, 100, 1000 y 10000 ug/L during 24 h. Biochemical parameters were measured espectrophotometrically. Peripheral blood smears, two per fish, were made by applying a drop of blood on clean slides, fixed in absolute methanol; air dried and stained with 15% Giemsa solution. Two thousand erythrocytes, 1000 per slide, were analyzed from each animal under 1000 X magnification. In gills GST and CAT activity was inhibited at 10, 100, 1000 ug/L and at 1, 10, 100, 1000 ug/L, respectively (p