Inflammatory response mediated by reactive oxygen species in neutrophils of inmunocompromised no-HIV infected patients

CARBIA, C; LAIRION F; FERNANDEZ, S; MERELLI A; TROITIÑO JM; LAZAROWSKI A; VELLICE, A; REPETTO MG

Congreso; Reunión Anual de Sociedades de Biociencia. SAIC, SAI, SAFIS.; 2020

Sociedad Argentina de Investigacion clinica

Immunocompromised patients (IP) with neutropenia (moderate (MN), less than 1500 neutrophils PMN)/mm3 and severe (SN), less than 500/mm3) generate susceptibility to infections. Neutropenia is prevalent in Leukemias (Le) and Lymphomas (Li). In order to analyze the inflammatory response the study was made on non-HIV IP with Le, Li and renal transplanted IP (RT), infected (I, no-HIV) and without infection (WI); it was determined in PMN venous blood from IP (n=49, 48±17 years, hospitalized), and healthy volunteer donors (C, n=30, 35±12 years): oxygen consumption (∆O2, Clark electrode, indicates generation of superoxide anion, O2-), production of hydrogen peroxide (H202, fluorometry), spontaneous chemiluminescence of PMN (CL, photon counter, measures light emission of singlet oxygen (1O2)), and C-reactive protein (CRP). Increases were observed in IP with respect to C: ∆O2 9 fold in WI and 3 in I (p