Impaired mitochondrial function correlates with heart, kidney and lung morphologycal alterations in acute copper overoads

ACOSTA, JUAN MANUEL; MORALES, CELINA; RODRÍGUEZ, MANUEL; LAIRION, FABIANA; GELPI, RICARDO; BOVERIS, ALBERTO; REPETTO, MARISA GABRIELA

Mar del Plata

Congreso; LXIII Reunión anual de la Sociedad Argentina de Investigación Clínica; 2018

Sociedad Argentina de Investigación Clínica

Oxidative damage (OD) and mitochondrial bioenergetic dysfunction are associated to acute overload of copper(II) preceding rat death. The aim of this research is to evaluate if morphological damage in liver, heart, lung and kidney correlates with dose and mitochondrial damage. Sprague Dawley male rats (200 g) received Cu(II) at dose of 5.0; 6.5 and 7.5 mg/kg (ip) and were sacrificed 1h and 6hafter treatment. The organs were excised and the samples were processed according to routine methods for obtaining histopathological preparations that were stained with hematoxylin-eosin. The congestion index (CI) was calculated for each organ and dose. Mitochondrial function (oxygen uptake, ΔO2) was evaluated using a Clark type oxygen electrode and mitochondria Complex I-III andII-III activity using spectrophotometric methods. Results indicated that organs presented different indexes of congestion. CI increased with dose, CI minor than 1 corresponds to dose 5 mg/kg; 0.2 to 1.2 (mild-moderate) to dose of 6.5 mg/kg; and CI higher than 2 (moderate to strong) to dose of 7.5 mg/kg (25% survival of rats). Activity of complex I-III and II-III decreased in heart (21%, p