Nrf2-ARE signaling pathway associated to antioxidant response in rat brain after acute iron and copper overloads

REPETTO MARISA; ACOSTA JUAN MANUEL; MUSACCO SEBIO ROSARIO; SAPORITO MAGRIÑÁ CRISTIAN; DAMIANO, ALICIA; CASTRO PARODI, MAURICIO; FUDA, JULIAN; TORTI, HORACIO; FERRAROTTI, NIDIA; BOVERIS ALBERTO

´París

Congreso; Paris Redox 2017; 2017

The Nrf2-ARE (nuclear factor erythroid 2 (NFE2)-related factor 2- antioxidant responsive element) signaling pathway is involved in detoxification and elimination of reactive oxidants through antioxidant and conjugation reactions and by enhancing cellular antioxidant content. The molecule that seems to be the signal for the genomic transcription is a soluble phospholipid hydroperoxide (ROOH). Rats (220 ± 8 g) were given single doses of 30 mg/kg iron (Fe) or 5 mg/kg copper (Cu). The activities and expressions of brain cytosolic superoxide dismutase (SOD1), catalase and levels of Nrf2 in brain were determined in the period of 0-24 h after metal ion loads. SOD1, catalase and Nrf2 increased their expressions in response to oxidative stress: SOD1, 2.5 and 1.8 times; catalase 2 and 2.5 times after 24 h (p