A HIGH THROUGHPUT APPROACH TO DELINEATE PROTEIN COMPLEXES IN FORMALIN- FIXED PARAFFIN-EMBEDDED PROSTATE ADENOCARCINOMA AND BENIGN PROSTATIC HYPERPLASIA TISSUES

JUAN BIZZOTTO; SOFIA LAGE VICKERS; PIA VALACCO,; SANCHIS, PABLO; OSVALDO MAZZA; CARLOS SCORTICATI; SERGIO NEMIROVSKY; COTIGNOLA JAVIER; ELBA VAZQUEZ.; GERALDINE GUERON

Mar del Plata

Congreso; LXIV Reunión anual de la Sociedad Argentina de Investigación Clínica; 2019

Sociedad Argentina de Investigación Clínica

Prostate cancer (PCa) is a progressivedisease involving multiple gene alterations; however, little isknown at the proteome level. Most of the functional informationof the cancer-associated genes relies in the proteome, a complexbiological system with dynamic interactions. To identifydifferential protein complexes associated with PCa we carried outan in-depth proteomics analysis. PCa and benign prostatichyperplasia (BPH) samples were obtained from the Hospital deClínicas ?José de San Martín? (with written informed consent andinstitutional review board approval). Proteins were obtainedusing phase-transfer surfactant-aided extraction/tryptic digestionof formalin-fixed, paraffin-embedded (FFPE) tissue sectionsmounted on microscope slides. Samples were subjected to massspectrometry analysis (ESI-MS/MS). We found 109 proteinsenriched in PCa compared with BPH samples. To identify proteincomplexes differentially expressed we used CORUM, a databaseof human protein complexes. Utilizing biological complexes as acluster vector, we calculated a proteomics signature profile foreach sample based on the hit rates of their reported proteins, inthe absence of fold change thresholds, against the cluster vector.We identified the following differentially expressed proteincomplexes in PCa compared with BPH (p