Effect of the medium Talp on the selection of pig spermatozoas through the use of hydrogels

BLOIS, DAMIÁN; BARBERO, CÉSAR A.; LIAUDAT, ANA C.; RIVAROLA, CLAUDIA R.; RODRÍGUEZ, NANCY; MORILLA, GRISELDA; CAPELLA, VIRGINIA; BOSCH, PABLO

Mar del Plata

Congreso; Reunión Anual de las Sociedades de Biociencias 2019; 2019

Sociedad Argentina de Biología (SAB)

In order to select sperm cells with high fertilizer quality, PNIPAM copolymerized with APTA-15 0/0 hydrogel surfaces were used. This biomaterial is becoming very important due to its low cellular toxicity, positive vet charge and its great versatility. The aim of this work was to evaluate the binding and release capacity of pig sperm to surfaces of PNIPAM co-APTA-15% in TALP-Ca++ and TALP- without Ca++. Initially, the effect of sperm manipulation on sperm viability of was evaluated by exposure to the TALP-Ca++ medium, without the presence of the hydrogel. In order to evaluate the effect of calcium on cell adhesion, pig sperm were exposed to PNIPAM co-APTA-15 0/0 hydrogels surfaces, in TALP-Ca++ and TALP- medium without Ca++ for 30. Subsequently the medium was replaced by TALP-Ca++ and TALP-without Ca++ in order to analyze whether the cation affects sperm cells relase from hydrogel. Results were statistically analyzed by one way ANOVA and Bonferroni as a post-hoc test (p