Response of macrophages in contact with synthetic biomaterials based on poly-N-isopropylacrylamide and copolymer hydrogels

LIAUDAT, ANA C.; RIVAROLA, CLAUDIA R.; RIVERO, REBECA E.; BOSCH, PABLO; CAPELLA, VIRGINIA; BARBERO, CÉSAR A.; RODRÍGUEZ, NANCY

CABA

Congreso; Reunión Anual de las Sociedades de Biociencias 2019; 2019

Sociedad Argentina de Biología (SAB)

Biomaterials are being developed in the last decades, in regenerative medicine field,to create tissue constructs that possess mechanical and physiological similarities with the tissue to be simulated. Hydrogels are one of the most promising materials due to their innate similarity with extracellular matrix (ECM), with mechanical properties adjustable and a good biocompatibility that allow cell adhesion and proliferation. Previously to use the biomaterial in vivoconditions, it is essential to study the macrophage-material interaction and consider it as a variable that influences the biocompatibility and the processes that govern tissue regeneration.The aim of this study was to analyze the response of macrophage RAW 264.7 in contact with biomaterials. Polymeric hydrogels based in poly-N-isopropylacrylamide with positive (3-acrylamidopropyl-trimethylammonium chloride, APTAC), negative (2-acrylamido-2-methylpropanesulfonic acid, AMPS) and neutral (N-acryloyl-tris-hydroximethylaminomethane, HMA) net charges were synthesized. MTT and neutral red uptake, nitric oxide quantification and attachment assays were performedat 1, 4 and 7 days of exposition, in order to assess cell viability and macrophage polarization,respectively.Cells without treatment were included as negative control. The resultsofcell viability and nitric oxide production did not differ among macrophages exposed to hydrogels and negative control (P>0.05).Cell adhesion and morphology varied according to the hydrogel charge net. These preliminary results indicate thatPNIPAM based hydrogels could be used in futures applications as cell scaffold for tissue-engineered construct.