ANTI-INFLAMMATORY ACTIVITY OF NEBULIZED CURCUMIN IN NANOVESICLES

ALTUBE MARÍA JULIA; LILEN IVONNE CAIMI; MARÍA JOSÉ MORILLA; ROMERO EDER LILIA

Mar del Plata

Conferencia; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

Curcumin (CUR) is a polyphenoliccompound obtained from the roots of Curcuma Longa with a widerange of therapeutic properties such as antioxidant and antiinflammatory activity. However, due to its hydrophobicity andinstability in aqueous solution, it has a poor oral absorption and arapid systemic elimination, which impede its clinical use.Archaeosomes (ARC) are nanovesicles made of archaeolipids thatresist chemical and enzymatic hydrolysis as well as oxidation.Moreover, archaeolipids are ligands of scavenger receptors sothey facilitate active targeting to cells that express thesereceptors in their surface. The aim of this work is to developnovel nanoformulations of CUR for the treatment of inflammatorylung diseases that can be nebulized and improved the activity ofthe drug. CUR was incorporated in lipidic bilayers of ARC(ARCUR) and of conventional liposomes (LCUR). Bothformulations of nanovesicles had sizes of nearly 150 nm thatwere successfully nebulized and lipid and CUR recovery up to 85%. To evaluate safety and efficacy aspects of nebulizednanoformulations we used a model of alveolar epithelium, whichconsisted on A549 cell line growing in transwells membranes inan air-liquid interface that were activated with LPS to releasepro-inflammatory cytokines. ARCUR could completely inhibit therelease of IL-8 and IL-6 while nanoLCUR could only completelyinhibit IL-6 release. Transepithelial electrical resistance (TEER)and lucifer yellow permeability across the monolayer (LYP) weremonitored because these are important parameters of theepithelial tightness. Monolayers activated with LPS decreasedTEER values and increased LYP, with co-incubation of ARCURthese values were restored to the control values, while LCUR didnot produced an inhibitory effect against LPS. In conclusion,ARCUR has potential for being used as therapeutic agent in lunginflammatory diseases.