Effect of Alkaline Gradient on Clear Renal Cell Carcinoma Proliferation: Role of Isoform 1 of Na+/H+ Exchanger Function.

ANA MECHALI; BELEN CABRAL; GISELA DI GIUSTO; BELTRAMONE, NATALIA; PAULA FORD; CLAUDIA CAPURRO; RIVAROLA, VALERIA

Congreso; Reunión Conjunta de Biociencias SAIC-SAI-SAFIS (2020); 2020

) EFFECT OF ALKALINE GRADIENT ON CLEARRENAL CELL CARCINOMA PROLIFERATION: ROLE OFISOFORM 1 OF NA+/H+ EXCHANGER FUNCTION.Mechali A, Cabral B, Di Giusto G, Beltramone N, Ford P, Capurro C, Rivarola VUniversidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Biomembranas.Buenos Aires, Argentina. CONICET-Universidad de BuenosAires. Instituto de Fisiología y Biofísica ?Bernardo Houssay?IFIBIO-HOUSSAY. Buenos Aires, Argentina. vrivarola@yahoo.comThe association between proliferation and intracellular pH elicitsthe possibility that extracellular pH (pHe) may modify cell survival.Moreover, as tumor extracellular acidity is a hallmark of cancer, isprobable that pHe affects differently cancer or normal cells. Our previous studies showed that cells derived from renal cell carcinoma(RCC) were more sensible to cell death after 72h exposition to 9.6mM NaOH (mild alkalosis) than normal cells. The aim of this studywas to investigate if this alkaline condition also affects cell proliferation. We use three renal cell models: HK2, derived from normal human proximal epithelial cells, 786-O and Caki-1, both derived fromhuman RCC. We exposed cells to media with 9.6 mM NaOH for72h. Then, we estimated cell proliferation by BrdU experiments. Ourresults show at pH 7.4 both RCC derived cell lines proliferate morethan normal HK2 cells (% BrdU+ cells: HK2= 31±2; 786-O= 46±3and Caki-1= 58±3, p