MATERNAL DIETS ENRICHED IN SUNFLOWER AND CHIA OIL MODULATE PPAR PATHWAYS IN THE DECIDUA FROM DIABETIC RATS DURING EARLY POSTIMPLANTATION

ROBERTI SABRINA L; SATO HUGO; GATTI CINTIA R; HIGA ROMINA; JAWERBAUM ALICIA

Congreso; International Federation of Placenta Associations (IFPA 2019); 2019

Objectives: PPARs are nutritional sensors whose activation regulates genesinvolved in lipid metabolism and transfer. Previously, in the decidua from9-day-pregnant diabetic rats we found that PPARgamma and its targetgenes PLIN2 and FABP4 were increased while PPARalpha and its targetgenes ACO and CPT-1 were reduced. Here, we hypothesized that maternaldiets enriched with sunflower and chia oil (Sources of n-6 and n-3 PUFAs,PPARs natural ligands) modulate PPARs levels and their target genes in thedecidua from 9-day-pregnant diabetic rats.Methods: Diabetes was induced in female rats by streptozotocin administration(50 mg/kg) two weeks before mating. On days 7 to 9 of pregnancy,diabetic rats received a standard diet or a diet enriched either in 6% ofsunflower oil or 6% chia oil. On day 9 of pregnancy the decidua wasexplanted, PPARs were measured by Western blot, target genes mRNAlevels by RT-qPCR and proteins expression by immunohistochemistry.Results: Maternal diets enriched in sunflower and chia oils prevented theincreased PPARgamma levels in the decidua from diabetic rats. AlthoughPLIN2 levels did not change with the maternal treatments, FABP4 levelswere increased in the decidua from diabetic rats fed the sunflower andchia oil-enriched diets (85% and 123%; p<0.01). Sunflower oil but not chiaoil enriched-diet, prevented the reduction of PPARalpha protein expressionand increased the mRNA expression of ACO and CPT-1 in the deciduafrom diabetic rats (90% and 74%; p<0.01)Conclusion: Diets enriched in n-6 and n-3 PUFAs prevented maternaldiabetesinduced increases in PPARgamma levels in the decidua duringearly postimplantation and upregulated proteins involved in lipid transfer.Only the diet enriched in n-6 PUFAs activated PPARalpha pathways in thedecidua from diabetic rats. These results highlight the dietary oils capacityto regulate and activate PPAR signaling in the decidua.