Decidual abnormalities in the prepubertal period and at day 9 of pregnancy in diabetic rat offspring

GATTI CINTIA R; ROBERTI SABRINA L; GOMEZ RIBOT DALMIRO; CAPOBIANCO EVANGELINA; JAWERBAUM ALICIA

Simposio; III Simposio Colombiano de Placenta e Interacción Materno-fetal; 2020

Introduction: Maternal diabetesinduces an intrauterine prooxidant/proinflammatory environment, associated toimpaired PPAR pathways, which could alter the offspring?s uterus. Objectives:To evaluate, in control and diabetic rat offspring, the uterine weight(decidualized or not) in the prepubertal period, and decidual PPARs proteinexpression on day 9 of pregnancy. Methods: Diabetes was induced in F0 rats byneonatal administration of streptozotocin (90 mg/kg sc). Females were matedwith healthy males to study the offspring?s uterus a) on postnatal day 30, withor without induction of decidualization (PMSG/hCG, 50 IU) and b) on day 9 ofpregnancy. Uteri were weighed at the prepubertal stage and decidual PPAR levelswere evaluated on day 9 of pregnancy. Results: In the prepubertal stage, theuterus of the offspring of diabetic rats showed reduced weight (p<0.05 vsControl), and the induction with PMSG/hCG increased its weight to valuessimilar to controls. In pregnancy, number of implantations and resorptions didnot change in the studied groups. Protein expression of PPARalpha, PPARgammaand PPARdelta were found reduced (p<0.05 vs Control). Conclusions: Maternaldiabetes alters the offspring?s uterus at prepuberty and leads to reducedexpression of decidual PPARs in pregnancy, possibly contributing to an F1 prooxidant/proinflammatoryintrauterine environment.