EFFECTS OF SHIGA TOXIN TYPE 2 IN PREGNANT AND NON-PREGNANT FEMALE RATS

FISCHER SIGEL, LILIAN KARINA; SACERDOTI, FLAVIA; IBARRA, CRISTINA; ZOTTA, ELSA; SILBERSTEIN, CLAUDIA

Congreso; Reunión Anual de Sociedades de Biociencias SAIC?SAI?SAFIS; 2020

Shiga toxin-producing Escherichia coli causes acute renal failure and Hemolytic Uremic Syndrome. It was reported that inhibition of nitric oxide (NO) by Shiga toxin type 2 (Stx2) enhanced renal damage in mice and baboon models of Stx-mediated HUS. The aim of the work was to study the evolution of the damages caused by Stx2 in P compare with NP rats. Pregnant Sprague-Dawley rats, at day 8 of gestation, and NP rats were ip inoculated with 0.5 ng Stx2/g body weight (PS, NPS) or diluent (PC, NPC). Some PS and PC rats were treated with 1mg/ml L-NAME, NO inhibitor, in drinkingwater (PLS, PLC) from 24h before ip injection to 4 days post-injection (dpi). Rats were individually housed, checked for water and food intake, and weighted every 24h until 30 dpi. At 4 dpi, bloodand 24h-urine samples were collected to determine urinary flow and free water clearance (CH20). Then, rats were euthanized and kidneys were removed for histopathological observations. NPS and PS rats showed a decrease in food intake and weight with respect to controls (p