SPECIFIC ISOTYPES OF THE RETINOIC ACID RECEPTOR (RARS) DIFFERENTIALLY MODULATE PARAMETERS ASSOCIATED WITH METASTATIC SPREAD IN TRIPLE NEGATIVE MAMMARY CANCER CELLS.

AMIGO NATALIA; CAÑONERO LUCIANA; ARIZA BAREÑO LIZETH; BECHIS ANDRES; DELBART DAMIAN; URTREGER ALEJANDRO; TODARO LAURA

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Congreso; ? Reunión Anual de Sociedades de Biociencia SAIC.SAI.SAFIS 2020.; 2020

SPECIFIC ISOTYPES OF THE RETINOIC ACID RECEPTOR (RARS) DIFFERENTIALLY MODULATE PARAMETERS ASSOCIATED WITH METASTATIC SPREAD IN TRIPLE NEGATIVE MAMMARY CANCER CELLS.Amigo N1, Cañonero L1, Ariza Bareño L1, Bechis A1, Delbart D1, Urtreger A1, Todaro L1.1. Departamento de Biología Celular. Área de Investigación. Instituto de Oncología "Ángel H. Roffo". Universidad de Buenos Aires.Migration and adhesion are highly related to metastatic dissemination and retinoid system is implicated in their modulation. Objective: Evaluate the effect of activating each retinoic acid receptor (RAR) isotype in migration, soluble MMPs activity, adhesion and metastatic potential in LM38-LP and 4T1 triple negative murine cell lines. Both cell lines express all RAR isotypes with the exception of RARβ in 4T1 cells.Cells were treated with the RARα agonist AM580 (200 nM), RARβ agonist AC55649 (2 μM), RARγ agonist BMS961 (50 nM) or vehicle (DMSO). Migratory potential was evaluated by wound healing assays. AM580 and AC55649 reduced LM38-LP migratory capacity (p